Differentiation of Mesenchymal Stem Cells Derived From Human Adipose Tissue Into Cholinergic-like Cells: An in Vitro Study.

ABSTRACT:

Introduction

Cholinergic-associated diseases currently constitute a significant cause of neurological and neurodegenerative disabilities. As the drugs are not efficient in improving the suffered tissues, stem cell treatment is considered an effective strategy for substituting the lost cells.

Methods

In the current study, we set out to investigate the differentiation properties of human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) into cholinergic-like cells by two morphogens of Retinoic Acid (RA) and Sonic Hedgehog (Shh) using a three-step in vitro procedure. The results were evaluated using real-time PCR, flow cytometry, and immunocytochemistry for two weeks.

Results

Our data showed that the cells could express cholinergic specific markers, including Islet-1, Ace

SUBMITTER: Sanooghi D 

PROVIDER: S-EPMC8666926 | biostudies-literature | 2021 May-Jun

REPOSITORIES: biostudies-literature