Iron-binding cellular profile of transferrin using label-free Raman hyperspectral imaging and singular value decomposition (SVD).

ABSTRACT: Serum transferrin (Tf) is the essential iron transport protein in the body. Transferrin is responsible for the sequestration of free iron in serum and the delivery of iron throughout the body and into cells, where iron is released inside a mildly acidified endosome. Altered iron distributions are associated with diseases such as iron-overload, cancer, and cardiovascular disease. The presence of free iron is linked to deleterious redox reactions, inside and outside cells and organelles. As Tf iron release is pH dependent, any changes in intraorganelle and extracellular pH, often associated with disease progression, could inhibit normal iron delivery or accelerate iron release in the wrong compartment. However, imaging approaches to monitor changes in the iron-bound state of Tf are lacking.

SUBMITTER: Tubbesing K 

PROVIDER: S-EPMC8667008 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature