Effect of cigarette smoke extract on mitochondrial division in mouse quadriceps femoris cells.

ABSTRACT:

Background

To observe the effect of cigarette smoke extract (CSE) on mitochondrial division in mouse quadriceps femoris cells and to explore the potential molecular mechanism of skeletal muscle dysfunction (SMD) in patients with chronic obstructive pulmonary disease (COPD).

Methods

Quadriceps femoris were cultured, passaged, and stimulated with different concentrations of CSE. We divided cells into four groups (Control, 2.5%, 5%, 10%). The growth of cells, the expression of Dynamin related protein 1 (Drp-1), and apoptosis were observed and evaluated by fluorescence microscopy, RT-PCR, Western blot, and flow cytometry.

Results

The longer the intervention time, the more obvious the decrease in cell number. In the 5% and 10% groups, the cells became round with gaps. Unde

SUBMITTER: Tan Z 

PROVIDER: S-EPMC8667143 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature