Y-box binding protein 1 regulates liver lipid metabolism by regulating the Wnt/β-catenin signaling pathway.

ABSTRACT:

Background

We mainly investigated how y-box binding protein 1 (YB-1) regulates liver lipid metabolism through the Wnt/β-catenin signaling pathway using multiple models.

Methods

The LO2 cells were treated with palmitic acid (PA) to create an NAFLD model in vitro. Immunohistochemistry and Western blotting assays were used to detect the expression of YB-1, β-catenin, SREBP-1c, LXRa, FXR1 and PPARα protein, and RNAs of them was detected by qRT-PCR. Oil Red O assay was applied to observe lipid droplets in LO2 cells and liver tissues. H&E staining was performed to observe the degree of liver inflammation. Proteomics in LO2 cells were conducted by Tandem mass tag proteomics assay. Co-immunoprecipitation and Western blotting assays we

SUBMITTER: Ma Z 

PROVIDER: S-EPMC8667161 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature