A pilot study of microbial signatures of liver disease in those with HIV mono-infection in Rio de Janeiro, Brazil.

ABSTRACT:

Objective

The rectal microbiome was examined to assess the relationship between the microbiome and liver disease in HIV-infection.

Design

Eighty-two HIV-1 mono-infected individuals from the PROSPEC-HIV-study (NCT02542020) were grouped into three liver health categories based on results of controlled attenuation parameter (CAP) and liver stiffness measurement (LSM) of transient elastography: normal (n = 30), steatosis (n = 30), or fibrosis (n = 22).

Methods

Liver steatosis and fibrosis were defined by CAP at least 248 dB/m and LSM at least 8.0 kPa, respectively. 16S rRNA gene and whole genome shotgun metagenomic sequencing were performed on rectal swabs. Bacterial differences were assessed using zero-inflated negative binomial regression and random forests modeling; ta

SUBMITTER: Yanavich C 

PROVIDER: S-EPMC8667204 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature