A rapid and non-destructive method for spatial-temporal quantification of colonization by Pseudomonas syringae pv. tomato DC3000 in Arabidopsis and tomato.

ABSTRACT: BACKGROUND:The bacterial leaf pathogen Pseudomonas syringae pv tomato (Pst) is the most popular model pathogen for plant pathology research. Previous methods to study the plant-Pst interactions rely on destructive quantification of Pst colonisation, which can be labour- and time-consuming and does not allow for spatial-temporal monitoring of the bacterial colonisation. Here, we describe a rapid and non-destructive method to quantify and visualise spatial-temporal colonisation by Pst in intact leaves of Arabidopsis and tomato. RESULTS:The method presented here uses a bioluminescent Pst DC3000 strain that constitutively expresses the luxCDABE operon from Photorhabdus luminescens (Pst::LUX) and requires a common gel documentation (Gel Doc) system with a sensitive CCD/CMOS camera and imaging s

SUBMITTER: Furci L 

PROVIDER: S-EPMC8667384 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature