Overexpression of fucosyltransferase 8 reverses the inhibitory effect of high-dose dexamethasone on osteogenic response of MC3T3-E1 preosteoblasts.

ABSTRACT:

Background

Core fucosylation catalyzed by FUT8 is essential for TGF-β binding to TGF-β receptors.

Methods

Indirect TGF-β1 binding assay was used to evaluate the ability of TGF-β1 to bind to TGFBRs, Alizarin red and alkaline phosphatase staining were used to detect osteogenic differentiation and mineralization ability , western blot and quantitative RT-PCR were used to measure the differential expression of osteogenesis-related proteins and genes. Plasmid-mediated gain-of-function study. The scale of core fucosylation modification was detected by Lectin-blot and LCA laser confocal.

Results

Our results showed that compared with vehicle treatment, high-dose (10^-6 and 10^-5 M) dexamethasone significantly inhibited cell proliferation, osteogenic differ

SUBMITTER: Wu Z 

PROVIDER: S-EPMC8667747 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature