Frequent, quantitative bone planar scintigraphy for determination of bone anabolism in growing mice.

ABSTRACT:

Background

To provide insight into bone turnover, quantitative measurements of bone remodeling are required. Radionuclide studies are widely used in clinical care, but have been rarely used in the exploration of the bone in preclinical studies. We describe a bone planar scintigraphy method for frequent assessment of bone activity in mice across the growing period. Since repeated venous radiotracer injections are hardly feasible in mice, we investigated the subcutaneous route.

Methods

Repeated ^99mTc-hydroxymethylene diphosphonate (HMDP) tracer bone planar scintigraphy studies of the knee region and µCT to measure femur growth rate were performed in eight mice between week 6 and week 27 of life, i.e., during their growth period. Three independent investigator

SUBMITTER: Zaloszyc A 

PROVIDER: S-EPMC8667748 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature