Using recombinase-mediated cassette exchange to engineer MIN6 insulin-secreting cells based on a newly identified safe harbor locus.

ABSTRACT:

Aims/introduction

Recent studies have identified genomic and transcript level changes along with alterations in insulin secretion in patients with diabetes and in rodent models of diabetes. It is important to establish an efficient system for testing functional consequences of these changes. We aimed to generate such a system using insulin-secreting MIN6 cells.

Materials and methods

MIN6 cells were first engineered to have a tetracycline-regulated expression system. Then, we used the recombination-mediated cassette exchange strategy to explore the silencing-resistant site in the genome and generated a master cell line based on this site.

Results

We identified a site 10.5 kbps upstream from the Zxdb gene as a locus that allows homogenous transgene expression from a tet

SUBMITTER: Furukawa A 

PROVIDER: S-EPMC8668067 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature