Bacterial vaginosis and health-associated bacteria modulate the immunometabolic landscape in 3D model of human cervix.

ABSTRACT: Bacterial vaginosis (BV) is an enigmatic polymicrobial condition characterized by a depletion of health-associated Lactobacillus and an overgrowth of anaerobes. Importantly, BV is linked to adverse gynecologic and obstetric outcomes: an increased risk of sexually transmitted infections, preterm birth, and cancer. We hypothesized that members of the cervicovaginal microbiota distinctly contribute to immunometabolic changes in the human cervix, leading to these sequelae. Our 3D epithelial cell model that recapitulates the human cervical epithelium was infected with clinical isolates of cervicovaginal bacteria, alone or as a polymicrobial community. We used Lactobacillus crispatus as a representative health-associated commensal and four common BV-associated species: Gardnerella vaginalis, Pre

SUBMITTER: Laniewski P 

PROVIDER: S-EPMC8669023 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature