Thiol profiling in cancer cell lines by HPLC-mass spectrometry.
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ABSTRACT:
SUBMITTER: Liu X
PROVIDER: S-EPMC8669100 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Thiol profiling in cancer cell lines by HPLC-mass spectrometry.
Liu Xiaoguang X Koppula Pranavi P Olszewski Kellen K Gan Boyi B
STAR protocols 20211208 4
We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Kopp ...[more]
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