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Differentiation of human pluripotent stem cells into pancreatic duct-like organoids.

ABSTRACT: The recapitulation of human developmental processes and pathological manifestations requires access to specific cell types and precursor stages during embryogenesis and disease. Here, we describe a scalable in vitro differentiation protocol to guide human pluripotent stem cells stepwise into pancreatic duct-like organoids. The protocol mimics pancreatic duct development and was successfully used to model the onset and progression of pancreatic ductal adenocarcinoma; the approach is suitable for multiple downstream applications. However, the protocol is cost- and time-intensive. For complete details on the use and execution of this protocol, please refer to Breunig et al. (2021).

SUBMITTER: Breunig M

PROVIDER: S-EPMC8669107 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Differentiation of human pluripotent stem cells into pancreatic duct-like organoids.

Breunig Markus M Merkle Jessica J Melzer Michael Karl MK Heller Sandra S Seufferlein Thomas T Meier Matthias M Hohwieler Meike M Kleger Alexander A

STAR protocols 20211208 4

The recapitulation of human developmental processes and pathological manifestations requires access to specific cell types and precursor stages during embryogenesis and disease. Here, we describe a scalable <i>in vitro</i> differentiation protocol to guide human pluripotent stem cells stepwise into pancreatic duct-like organoids. The protocol mimics pancreatic duct development and was successfully used to model the onset and progression of pancreatic ductal adenocarcinoma; the approach is suitab ...[more]

PMID: 34917972

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