SdhA blocks disruption of the Legionella-containing vacuole by hijacking the OCRL phosphatase.

ABSTRACT: Legionella pneumophila grows intracellularly within a replication vacuole via action of Icm/Dot-secreted proteins. One such protein, SdhA, maintains the integrity of the vacuolar membrane, thereby preventing cytoplasmic degradation of bacteria. We show here that SdhA binds and blocks the action of OCRL (OculoCerebroRenal syndrome of Lowe), an inositol 5-phosphatase pivotal for controlling endosomal dynamics. OCRL depletion results in enhanced vacuole integrity and intracellular growth of a sdhA mutant, consistent with OCRL participating in vacuole disruption. Overexpressed SdhA alters OCRL function, enlarging endosomes, driving endosomal accumulation of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P₂), and interfering with endosomal trafficking. SdhA interrupts Rab guanosine tr

SUBMITTER: Choi WY 

PROVIDER: S-EPMC8669613 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature