Human and Animal RNA Virus Diversity Detected by Metagenomics in Cameroonian Clams.

ABSTRACT: Many recent pandemics have been recognized as zoonotic viral diseases. While their origins remain frequently unknown, environmental contamination may play an important role in emergence. Thus, being able to describe the viral diversity in environmental samples contributes to understand the key issues in zoonotic transmission. This work describes the use of a metagenomic approach to assess the diversity of eukaryotic RNA viruses in river clams and identify sequences from human or potentially zoonotic viruses. Clam samples collected over 2years were first screened for the presence of norovirus to verify human contamination. Selected samples were analyzed using metagenomics, including a capture of sequences from viral families infecting vertebrates (VirCapSeq-VERT) before Illumina NovaSeq seq

SUBMITTER: Bonny P 

PROVIDER: S-EPMC8669915 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature