Modeling ischemic stroke in a triculture neurovascular unit on-a-chip.

ABSTRACT:

Background

In ischemic stroke, the function of the cerebral vasculature is impaired. This vascular structure is formed by the so-called neurovascular unit (NVU). A better understanding of the mechanisms involved in NVU dysfunction and recovery may lead to new insights for the development of highly sought therapeutic approaches. To date, there remains an unmet need for complex human in vitro models of the NVU to study ischemic events seen in the human brain.

Methods

We here describe the development of a human NVU on-a-chip model using a platform that allows culture of 40 chips in parallel. The model comprises a perfused vessel of primary human brain endothelial cells in co-culture with induced pluripotent stem cell derived astrocytes and neurons. Ischemic stroke was mimicked

SUBMITTER: Wevers NR 

PROVIDER: S-EPMC8670153 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature