Linking function to global and local dynamics in an elevator-type transporter.

ABSTRACT: Transporters cycle through large structural changes to translocate molecules across biological membranes. The temporal relationships between these changes and function, and the molecular properties setting their rates, determine transport efficiency-yet remain mostly unknown. Using single-molecule fluorescence microscopy, we compare the timing of conformational transitions and substrate uptake in the elevator-type transporter Glt_Ph We show that the elevator-like movements of the substrate-loaded transport domain across membranes and substrate release are kinetically heterogeneous, with rates varying by orders of magnitude between individual molecules. Mutations increasing the frequency of elevator transitions and reducing substrate affinity diminish transport rate heterogeneitie

SUBMITTER: Ciftci D 

PROVIDER: S-EPMC8670510 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature