Vascular Endothelial-Breast Epithelial Cell Coculture Model Created from 3D Cell Structures.

ABSTRACT: Endothelial cell interactions with normal and cancerous breast epithelial cells have been widely studied in tissue growth and development, as well as in angiogenesis and metastasis. Despite the understanding that 3D multicellular architecture is critical to the cell phenotype, 3D vascular structures have not yet been cocultured with 3D breast spheroids in vitro. The objective of this study was therefore to create a hierarchical, multiscale model of vascular endothelial-breast epithelial cell interactions in which both cell types were assembled into their 3D architectures. The model was successfully fabricated by adding preformed breast spheroids onto preformed endothelial tube-like networks. Through this model, we observed that breast spheroids maintain vascular tube-like networks.

SUBMITTER: Swaminathan S 

PROVIDER: S-EPMC8670894 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature