Exercise prevents fatty liver by modifying the compensatory response of mitochondrial metabolism to excess substrate availability.

ABSTRACT:

Objective

Liver mitochondria adapt to high-calorie intake. We investigated how exercise alters the early compensatory response of mitochondria, thus preventing fatty liver disease as a long-term consequence of overnutrition.

Methods

We compared the effects of a steatogenic high-energy diet (HED) for six weeks on mitochondrial metabolism of sedentary and treadmill-trained C57BL/6N mice. We applied multi-OMICs analyses to study the alterations in the proteome, transcriptome, and lipids in isolated mitochondria of liver and skeletal muscle as well as in whole tissue and examined the functional consequences by high-resolution respirometry.

Results

HED increased the respiratory capacity of isolated liver mitochondria, both in sedentary and in trained mice. However, proteom

SUBMITTER: Hoene M 

PROVIDER: S-EPMC8671118 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature