Characterization of the liver immune microenvironment in liver biopsies from patients with chronic HBV infection.

ABSTRACT:

Background & aims

We aim to describe the liver immune microenvironment by analyzing liver biopsies from patients with chronic HBV infection (CHB). Host immune cell signatures and their corresponding localization were characterized by analyzing the intrahepatic transcriptome in combination with a custom multiplex immunofluorescence panel.

Method

Matching FFPE and fresh frozen liver biopsies were collected from immune active patients within the open-label phase IV study GS-US-174-0149. RNA-Seq was conducted on 53 CHB liver biopsies from 46 patients. Twenty-eight of the 53 samples had matched FFPE biopsies and were stained with a 12-plex panel including cell segmentation, immune and viral biomarkers. Corresponding serum samples were screened using the MSD Human V-plex Screen Se

SUBMITTER: van Buuren N 

PROVIDER: S-EPMC8671126 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature