Small volume retinol binding protein measurement by liquid chromatography-tandem mass spectrometry.

ABSTRACT:

Background

The measurement of plasma concentrations of retinol binding protein is a component of nutritional assessment in neonatal intensive care. However, serial testing in newborns is hampered by the limited amount of blood that can be sampled. Limitations are most severe with preterm infants, for whom close monitoring may be most important.

Methods

We developed an assay to quantify retinol binding protein using trypsin digestion and liquid chromatography-tandem mass spectrometry, which requires a serum or plasma volume of 5 µl. Additionally, we validated the method according to current recommendations and performed comparison with a standard nephelometry platform in clinical use.

Results

The assay demonstrated linearity from below 1 mg/dL (0.48 µM) to more than 20

SUBMITTER: Phipps WS 

PROVIDER: S-EPMC8671195 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature