Interleukin-5 receptor alpha (CD125) expression on human blood and lung neutrophils.

ABSTRACT:

Background

Our previous studies revealed the presence of interleukin-5 (IL-5) receptor alpha chain (IL-5Rα, CD125) on neutrophils in a murine model of influenza and in the lung fluid of children with severe asthma.

Objective

To further evaluate the functional characteristics and effects of clinical factors and inflammatory variables on neutrophil surface IL-5Rα abundance in lung fluid and blood.

Methods

IL-5Rα expression was quantified by flow cytometry performed on purified neutrophils from blood and bronchoalveolar lavage fluid samples obtained from healthy controls and individuals with asthma. Expression was further confirmed by immunohistochemistry. Functional signaling through the IL-5Rα was evaluated by measurement of IL-5-inducible modulation of neutrophil surf

SUBMITTER: Lawrence MG 

PROVIDER: S-EPMC8671196 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature