NAD(P)H autofluorescence lifetime imaging enables single cell analyses of cellular metabolism of osteoblasts in vitro and in vivo via two-photon microscopy.

ABSTRACT: Two-photon fluorescence lifetime microscopy (2P-FLIM) is a non-invasive optical technique that can obtain cellular metabolism information based on the intrinsic autofluorescence lifetimes of free and enzyme-bound NAD(P)H, which reflect the metabolic state of single cells within the native microenvironment of the living tissue. NAD(P)H 2P-FLIM was initially performed in bone marrow stromal cell (BMSC) cultures established from Col (I) 2.3GFP or OSX-mCherry mouse models, in which osteoblastic lineage cells were labelled with green or red fluorescence protein, respectively. Measurement of the mean NAD(P)H lifetime, τ_M, demonstrated that osteoblasts in osteogenic media had a progressively increased τ_M compared to cells in regular media, suggesting that osteoblasts undergo

SUBMITTER: Schilling K 

PROVIDER: S-EPMC8671374 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature