Polyphenol effects on CuO-nanoparticle-mediated DNA damage, reactive oxygen species generation, and fibroblast cell death.

ABSTRACT: The ability of ten polyphenolic antioxidants to prevent CuO nanoparticle (^NPCuO) and H₂O₂-mediated DNA damage and cytotoxicity was investigated. Five of the polyphenols (MEPCA, PREGA, MEGA, ECG, and EGCG) prevent ^NPCuO/H₂O₂-mediated DNA damage (IC₅₀ values of 7.5-800 μM), three have no effect (PCA, VA, and EC), and two (GA and EGC) result in increased DNA damage. Most polyphenols had similar antioxidant/prooxidant activity in the presence of ^NPCuO or free copper ions. Electron paramagnetic resonance (EPR) spectroscopy of reactive oxygen species (ROS) generated by ^NPCuO/H₂O₂ in the presence of representative polyphenols correlate with results of DNA damage studies: in the

SUBMITTER: Angele-Martinez C 

PROVIDER: S-EPMC8671380 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature