Optimised techniques for high-throughput screening of differentiated SH-SY5Y cells and application for neurite outgrowth assays.

ABSTRACT: Neuronal models are a crucial tool in neuroscientific research, helping to elucidate the molecular and cellular processes involved in disorders of the nervous system. Adapting these models to a high-throughput format enables simultaneous screening of multiple agents within a single assay. SH-SY5Y cells have been widely used as a neuronal model, yet commonly in an undifferentiated state that is not representative of mature neurons. Differentiation of the SH-SY5Y cells is a necessary step to obtain cells that express mature neuronal markers. Despite this understanding, the absence of a standardised protocol has limited the use of differentiated SH-SY5Y cells in high-throughput assay formats. Here, we describe techniques to differentiate and re-plate SH-SY5Y cells within a 96-well plate for h

SUBMITTER: Dravid A 

PROVIDER: S-EPMC8671469 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature