Optimization of critical parameters for coating of polymeric nanoparticles with plasma membrane vesicles by sonication.

ABSTRACT: Top-down functionalization of nanoparticles with cellular membranes imparts nanoparticles with enhanced bio-interfacing capabilities. Initial methods for membrane coating involved physical co-extrusion of nanoparticles and membrane vesicles through a porous membrane; however, recent works employ sonication as the disruptive force to reform membranes around the surface of nanoparticles. Although sonication is widely used, there remains a paucity of information on the effects of sonication variables on coating efficiency, leading to inconsistent membrane coating across studies. In this work, we present a systematic analysis of the sonication parameters that influence the membrane coating. The results showed that sonication amplitude, time, temperature, membrane ratio, sample volume, and dens

SUBMITTER: Yang F 

PROVIDER: S-EPMC8671476 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature