Differentiation of cancer stem cells into erythroblasts in the presence of CoCl₂.

ABSTRACT: Cancer stem cells (CSCs) are subpopulations in the malignant tumors that show self-renewal and multilineage differentiation into tumor microenvironment components that drive tumor growth and heterogeneity. In previous studies, our group succeeded in producing a CSC model by treating mouse induced pluripotent stem cells. In the current study, we investigated the potential of CSC differentiation into blood cells under chemical hypoxic conditions using CoCl₂. CSCs and miPS-LLCcm cells were cultured for 1 to 7 days in the presence of CoCl₂, and the expression of VEGFR1/2, Runx1, c-kit, CD31, CD34, and TER-119 was assessed by RT-qPCR, Western blotting and flow cytometry together with Wright-Giemsa staining and immunocytochemistry. CoCl₂ induced significant accum

SUBMITTER: Kumon K 

PROVIDER: S-EPMC8671479 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature