The distinct effects of P18 overexpression on different stages of hematopoiesis involve TGF-β and NF-κB signaling.

ABSTRACT: Deficiency of P18 can significantly improve the self-renewal potential of hematopoietic stem cells (HSC) and the success of long-term engraftment. However, the effects of P18 overexpression, which is involved in the inhibitory effects of RUNX1b at the early stage of hematopoiesis, have not been examined in detail. In this study, we established inducible P18/hESC lines and monitored the effects of P18 overexpression on hematopoietic differentiation. Induction of P18 from day 0 (D0) dramatically decreased production of CD34^highCD43- cells and derivative populations, but not that of CD34^lowCD43- cells, changed the cell cycle status and apoptosis of KDR+ cells and downregulated the key hematopoietic genes at D4, which might cause the severe blockage of hematopoietic diffe

SUBMITTER: Yi D 

PROVIDER: S-EPMC8671498 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature