A high-resolution mass spectrometry based proteomic dataset of human regulatory T cells.

ABSTRACT: Regulatory T cells (Tregs) play a core role in maintaining immune tolerance, homeostasis, and host health. High-resolution analysis of the Treg proteome is required to identify enriched biological processes and pathways distinct to this important immune cell lineage. We present a comprehensive proteomic dataset of Tregs paired with conventional CD4⁺ (Conv CD4⁺) T cells in healthy individuals. Tregs and Conv CD4⁺ T cells were sorted to high purity using dual magnetic bead-based and flow cytometry-based methodologies. Proteins were trypsin-digested and analysed using label-free data-dependent acquisition mass spectrometry (DDA-MS) followed by label free quantitation (LFQ) proteomics analysis using MaxQuant software. Approximately 4,000 T cell proteins were id

SUBMITTER: Weerakoon H 

PROVIDER: S-EPMC8671522 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature