Ontology highlight
ABSTRACT:
SUBMITTER: Chialastri A
PROVIDER: S-EPMC8672100 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
STAR protocols 20211211 4
The asymmetric distribution of 5-hydroxymethylcytosine (5hmC) between two DNA strands of a chromosome enables endogenous reconstruction of cellular lineages at an individual-cell-division resolution. Further, when integrated with data on genomic variants to infer clonal lineages, this combinatorial information accurately reconstructs larger lineage trees. Here, we provide a detailed protocol for single-cell 5-hydroxymethylcytosine and genomic DNA sequencing (scH&G-seq) to simultaneously quantify ...[more]