Ganglioside isomer analysis using ion polarity switching liquid chromatography-tandem mass spectrometry.

ABSTRACT: Gangliosides are ubiquitously present on cell surface. They are more abundantly expressed in nerve cells and tissues and involved in pathology of various diseases. Diversity of molecular structures in the carbohydrate head group, fatty acyl, and long chain base increases the complexity of analyzing gangliosides. In this study, an ultrahigh-performance liquid chromatography-tandem mass spectrometry method is developed for analysis of the co-eluting ganglioside isomers, which uses ion polarity switching to integrate glycan head isomer identification, ceramide isomer differentiation, and quantification of ganglioside into one analysis. The method is facilitated with an extensive ganglioside target list by combining the various glycan head groups, long chain bases, and the experimentally deter

SUBMITTER: Li Z 

PROVIDER: S-EPMC8672327 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature