Aptasensor based on fluorophore-quencher nano-pair and smartphone spectrum reader for on-site quantification of multi-pesticides.

ABSTRACT: Current techniques for the detection of multi-pesticides are limited by technical complexity, sensitivity and cost. There is an urgent demand of developing new specific recognition elements and sensitive signal readouts for on-site monitoring. In this work, we developed a fluorescent aptamer-based lateral flow biosensor (apta-LFB) integrated with fluorophore-quencher nano-pairs and a smartphone spectrum reader to accomplish triple-target detection of chlorpyrifos, diazinon, and malathion. Aptamers serve as alternative recognition elements instead of antibodies in LFB, offering better specificity and stability. A novel fluorophore-quencher nano-pair (quantum dots nanobeads and gold nanostars) was implemented to perform "signal-on" instead of "signal-off". After optimization, detection limit

SUBMITTER: Cheng N 

PROVIDER: S-EPMC8672368 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature