Dual-Readout Immunochromatographic Assay by Utilizing MnO₂ Nanoflowers as the Unique Colorimetric/Chemiluminescent Probe.

ABSTRACT: Manganese dioxide nanoflowers (MnO₂ NFs) were synthesized and used as a dual readout probe to develop a novel immunochromatographic test strip (ITS) for detecting pesticide residues using chlorpyrifos as the model analyte. MnO₂ NFs-labeled antibody for chlorpyrifos was employed as the signal tracer for conducting the ITS. After 10 min competitive immunoreaction, the tracer antibody was captured by the immobilized immunogen in the test strip, resulting in the captured MnO₂ NFs on test line. The captured MnO₂ NFs led to the appearance of brown color on the test line, which could be easily observed by the naked eye as a qualitative readout. Due to the very slight colorimetric difference of chlorpyrifos at trace concentrations, the semiquantitative r

SUBMITTER: Ouyang H 

PROVIDER: S-EPMC8672369 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature