WNT11/ROR2 signaling is associated with tumor invasion and poor survival in breast cancer.

ABSTRACT:

Background

Breast cancer has been associated with activation of the WNT signaling pathway, although no driver mutations in WNT genes have been found yet. Instead, a high expression of the alternative WNT receptor ROR2 was observed, in particular in breast cancer brain metastases. However, its respective ligand and downstream signaling in this context remained unknown.

Methods

We modulated the expression of ROR2 in human breast cancer cells and characterized their gene and protein expression by RNA-Seq, qRT-PCR, immunoblots and reverse phase protein array (RPPA) combined with network analyses to understand the molecular basis of ROR2 signaling in breast cancer. Using co-immunoprecipitations, we verified the interaction of ROR2 with the identified ligand, WNT11. The functional

SUBMITTER: Menck K 

PROVIDER: S-EPMC8672621 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature