Regulation of C. elegans L4 cuticle collagen genes by the heterochronic protein LIN-29.

ABSTRACT: The cuticle, the outer covering of the nematode C. elegans, is synthesized five times during the worm's life by the underlying hypodermis. Cuticle collagens, the major cuticle component, are encoded by a large family of col genes and, interestingly, many of these genes express predominantly at a single developmental stage. This temporal preference motivated us to investigate the mechanisms underlying col gene expression and here we focus on a subset of col genes expressed in the L4 stage. We identified minimal promoter regions of <300 bp for col-38, col-49, and col-63. In these regions, we predicted cis-regulatory sequences and evaluated their function in vivo via mutagenesis of a col-38p::yfp reporter. We used RNAi to study the requirement for candidate transcription regulators ELT-1 and

SUBMITTER: Abete-Luzi P 

PROVIDER: S-EPMC8672679 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature