Developing RT-LAMP assays for rapid diagnosis of SARS-CoV-2 in saliva.

ABSTRACT:

Background

The coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has killed millions of people worldwide. The current crisis has created an unprecedented demand for rapid test of SARS-CoV-2 infection.

Methods

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a fast and convenient method to amplify and identify the transcripts of a targeted pathogen. However, the sensitivity and specificity of RT-LAMP were generally regarded as inferior when compared with the gold standard RT-qPCR. To address this issue, we combined bioinformatic and experimental analyses to improve the assay performance for COVID-19 diagnosis.

Findings

First, by experimental screening as well as high-throughput sequencing studies, we discovered new primer features that i

SUBMITTER: Huang X 

PROVIDER: S-EPMC8674011 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature