Tryptase Regulates the Epigenetic Modification of Core Histones in Mast Cell Leukemia Cells.

ABSTRACT: Mast cells are immune cells that store large amounts of mast cell-restricted proteases in their secretory granules, including tryptase, chymase and carboxypeptidase A3. In mouse mast cells, it has been shown that tryptase, in addition to its canonical location in secretory granules, can be found in the nuclear compartment where it can impact on core histones. Here we asked whether tryptase can execute core histone processing in human mast cell leukemia cells, and whether tryptase thereby can affect the epigenetic modification of core histones. Our findings reveal that triggering of cell death in HMC-1 mast cell leukemia cells is associated with extensive cleavage of core histone 3 (H3) and more restricted cleavage of H2B. Tryptase inhibition caused a complete blockade of such processing. O

SUBMITTER: Alanazi S 

PROVIDER: S-EPMC8674432 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature