Microglia Impede Oligodendrocyte Generation in Aged Brain.

ABSTRACT:

Purpose

Age-related increase in myelin loss may be responsible for brain atrophy, and the mechanism is not completely understood. We aim to comprehensively delineate oligodendrocyte heterogeneity in young and aged mice and to reveal the underlying mechanism for myelin loss during aging.

Methods

Diffusion tensor imaging and immunofluorescent staining were performed to verify the demyelination in the aged brains of both rodents and human. Further, the single-cell RNA sequencing data of all brain cells from young and aged mice were deeply analyzed to identify the subsets of oligodendrocyte lineage cells. Cell-to-cell interaction analysis was performed to detect the mechanism of observed changes in oligodendrocyte generation.

Results

Oligodendrocytes were observed to up-r

SUBMITTER: Luan W 

PROVIDER: S-EPMC8674668 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature