Dataset Information

Function and transcriptional regulation of TCTN1 in oral squamous cell carcinoma.

ABSTRACT: Oral squamous cell carcinoma (OSCC) is one of the most common types of head and neck squamous cell carcinoma (HNSCC) with a poor survival rate. In the present study, the involvement of tectonic 1 (TCTN1) in OSCC was explored. The relevance between TCTN1 and HNSCC clinicopathological features was first analyzed and it was revealed that TCTN1 was associated with the tumor clinical stage and grade. In in vitro experiments, it was demonstrated that the proliferative, migratory and invasive capacity of OSCC CAL27 cells and SCC15 cells was significantly suppressed due to TCTN1 knockdown. Additionally, the core promoter of TCTN1 was confirmed and transcription factor AP‑2 alpha (TFAP2A) was suggested as a regulator of TCTN1 mRNA expression. On the whole, the present study elucidated the direct association between TCTN1 and OSCC for the first time, to the best of our knowledge, and the TFAP2A/TCTN1 axis was suggested as a potential novel therapeutic target for OSCC.

SUBMITTER: Bai G

PROVIDER: S-EPMC8674709 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Function and transcriptional regulation of TCTN1 in oral squamous cell carcinoma.

Bai Guangliang G Wei Ning N Li Fang F Zhao Peng P Meng Zhen Z Zou Bo B Liu Yujiao Y Xu Kai K Li Keyi K Yao Chao C Yang Peng P

Oncology reports 20211203 2

Oral squamous cell carcinoma (OSCC) is one of the most common types of head and neck squamous cell carcinoma (HNSCC) with a poor survival rate. In the present study, the involvement of tectonic 1 (TCTN1) in OSCC was explored. The relevance between TCTN1 and HNSCC clinicopathological features was first analyzed and it was revealed that TCTN1 was associated with the tumor clinical stage and grade. In <i>in vitro</i> experiments, it was demonstrated that the proliferative, migratory and invasive ca ...[more]

PMID: 34859261

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Project description:BackgroundResults of previous studies suggest that NANOG may be an important prognostic biomarker in oral squamous cell carcinoma (OSCC), but there are contradictory results regarding NANOG expression patterns on mRNA and protein levels, and the mechanisms of its regulation are poorly understood. Our aim was to analyze the expression and diagnostic significance of NANOG in OSCC, and the possible mechanisms of its regulation, i.e., protein regulators on mRNA level (OCT4, SOX2, KLF4, AGR2, and NOTCH1), methylation status, copy number variation, and regulatory miRNAs, miR-145, miR-335, miR-150, miR-34a, miR-128, and miR-27a.MethodsOur study included 120 patients with OSCC. Expression of NANOG protein and mRNA was analyzed using immunohistochemistry and qPCR. Expression of regulatory factors, miRNAs, and copy number variation was performed using qPCR. Methylation status of NANOG promoter was determined using PCR and Sanger sequencing.ResultsWe detected upregulation of NANOG and OCT4 and downregulation of NOTCH1 and AGR2 mRNA in OSCC with lymph node metastases compared to OSCC without lymph node metastases. We observed a strong positive correlation between mRNAs of NANOG and those of its protein regulators OCT4, SOX2, NOTCH1, AGR2, and KLF4. The expression of NANOG was in positive correlation with the expression of miR-34a. There was also a correlation between T status of OSCC and the expression of miR-335 and miR-150 and a correlation of miR-150 with the N status of T2 OSCC. NANOG promoter methylation and copy number variation were only observed in a small proportion of samples.ConclusionsOur findings confirm the diagnostic significance of NANOG in OSCC and provide information on NANOG expression patterns on both mRNA and protein levels. They also suggest that protein regulators and microRNAs might play a crucial role, whereas methylation of its promoter and copy number variation probably have a minor role in the regulation of NANOG expression in OSCC.

Dataset Information

Function and transcriptional regulation of TCTN1 in oral squamous cell carcinoma.

Publications

Function and transcriptional regulation of TCTN1 in oral squamous cell carcinoma.

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