A single-cell morpho-transcriptomic map of brassinosteroid action in the Arabidopsis root.

ABSTRACT: The effects of brassinosteroid signaling on shoot and root development have been characterized in great detail but a simple consistent positive or negative impact on a basic cellular parameter was not identified. In this study, we combined digital 3D single-cell shape analysis and single-cell mRNA sequencing to characterize root meristems and mature root segments of brassinosteroid-blind mutants and wild type. The resultant datasets demonstrate that brassinosteroid signaling affects neither cell volume nor cell proliferation capacity. Instead, brassinosteroid signaling is essential for the precise orientation of cell division planes and the extent and timing of anisotropic cell expansion. Moreover, we found that the cell-aligning effects of brassinosteroid signaling can propagate to normal

SUBMITTER: Graeff M 

PROVIDER: S-EPMC8674818 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature