Rapid Detection of bla _KPC, bla _NDM, bla _OXA-48-like and bla _IMP Carbapenemases in Enterobacterales Using Recombinase Polymerase Amplification Combined With Lateral Flow Strip.

ABSTRACT: The emergence of carbapenemase-producing Enterobacterales (CPE) infections is a major global public health threat. Rapid and accurate detection of pathogenic bacteria is essential to optimize treatment and timely avoid further transmission of these bacteria. Here, we aimed to develop a rapid on site visualization detection method for CPE using improved recombinase polymerase amplification (RPA) combined with lateral flow strip (LFS) method, based on four most popular carbapenemase genes: bla _KPC, bla _NDM, bla _OXA-48-like, and bla _IMP. All available allelic variants of the above carbapenemases were downloaded from the β-lactamase database, and the conserved regions were used as targets for RPA assay. Five primer

SUBMITTER: Wang F 

PROVIDER: S-EPMC8674914 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature