Genome-wide identification of enhancers and transcription factors regulating the myogenic differentiation of bovine satellite cells.

ABSTRACT:

Background

Satellite cells are the myogenic precursor cells in adult skeletal muscle. The objective of this study was to identify enhancers and transcription factors that regulate gene expression during the differentiation of bovine satellite cells into myotubes.

Results

Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) was performed to identify genomic regions where lysine 27 of H3 histone is acetylated (H3K27ac), i.e., active enhancers, from bovine satellite cells before and during differentiation into myotubes. A total of 19,027 and 47,669 H3K27ac-marked enhancers were consistently identified from two biological replicates of before- and during-differentiation bovine satellite cells, respectively. Of these enhancers, 5882 were specific to before-differe

SUBMITTER: Lyu P 

PROVIDER: S-EPMC8675486 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature