Using the co-expression network of T cell-activation-related genes to assess the disease activity in Takayasu's arteritis patients.

ABSTRACT:

Background

There have been lacking reliable serum biomarkers in assessing the disease activity of Takayasu's arteritis (TAK). This study aimed to assess the disease activity of TAK by assayed gene expression levels in peripheral mononuclear cells (PBMCs).

Methods

The expression level of genes that essential in T cell activation in PBMCs in active TAK patients, inactive TAK patients, and healthy controls were detected by real-time fluorescence quantitative polymerase chain reaction, including TCR, CD28, CD40, CD40L, PD-1, PD-L1, PD-L2, CTLA4, TIGIT, TIM3, LAG3, CCL5, T-bet, RORC, and FOXP3. Gene co-expression network was established, and the signature of the topology structure in active TAK patients compared to the inactive TAK patients were extracted and described by formula

SUBMITTER: Tian Y 

PROVIDER: S-EPMC8675511 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature