Immobilisation of phytase producing Gluconacetobacter with bacterial cellulose nano‐fibres and promotion of enzyme activities by magnetite nanoparticles

ABSTRACT: The isolated Gluconacetobacter sp. with accession number: KY996741 was assayed for evaluation of phytase activity. It could solubilise sodium phytate in the absence of soluble phosphate with the cells; however, the enzyme was not seen in cell free extract, to the best of their knowledge the intracellular phytase activities of Gluconacetobacter sp. was not reported previously. Also, the potential of in situ immobilisation of cells produced enzyme (/phytase producing bacteria) in bacterial cellulose was investigated and was studied by SEM and AFM. The results showed that the immobilised probiotic cells had the best activity of 1229 U/ml. The optimum temperature of the immobilised enzyme activity was at 45°C (5969 U/ml) and the immobilised phytase maintained 64% of its activities after two re

SUBMITTER: Jalili Tabaii M 

PROVIDER: S-EPMC8676266 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature