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Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated Bacillus vallismortis MDU6 strain.

ABSTRACT: Microbial biosurfactants has evolved as green molecules and their chemical diversity has gained momentum in recent time not only in the field of environmental and industrial sectors but also in the pharmaceutical sector. In this study, an effort was made for the biosynthesis of silver nanoparticle (AgNPs) having antimicrobial and non-cytotoxic activities with the help of microbial biosurfactant extracted from a novel Bacillus vallismortis strain MDU6 (Genbank accession no. MH382951) from petroleum oil logged soil sample in Dibrugarh, Assam. The isolate shows excellent potential for the production of biosurfactant by reducing the surface tension of diesel supplemented medium up to 56.57% only within 5 days. FTIR spectra of the crude biosurfactant show the presence of ʋ_CH2 (asymmetric stretching), ʋ_CH2 (symmetric stretching), ʋ_C=C (stretch), ʋ_C-C (stretch), ʋ_C-H (bending), ʋ_C-O (stretch) and ʋ_C-H (bending) functional groups and LC-MS/MS analysis confirms it as a cyclic lipopeptide which is a mixture of surfactin and iturin. The synthesized AgNPs showed excellent antimicrobial activities against Escherichia coli (ATCC no. 25922), Listeria monocytogenes (ATCC No. BAA-751), Staphylococcus aureus (ATCC No. 9542) and Bacillus subtilis (ATCC no. 6051) and showed no cytotoxicity against primary mouse liver cell lines.

SUBMITTER: Das M

PROVIDER: S-EPMC8676646 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated <i>Bacillus vallismortis</i> MDU6 strain.

Das Moonjit M Borah Debajit D Patowary Kaustuvmani K Borah Mayuri M Khataniar Ankita A Bhusan Kakoti Bibhuti B

IET nanobiotechnology 20191201 9

Microbial biosurfactants has evolved as green molecules and their chemical diversity has gained momentum in recent time not only in the field of environmental and industrial sectors but also in the pharmaceutical sector. In this study, an effort was made for the biosynthesis of silver nanoparticle (AgNPs) having antimicrobial and non-cytotoxic activities with the help of microbial biosurfactant extracted from a novel <i>Bacillus vallismortis</i> strain MDU6 (Genbank accession no. MH382951) from ...[more]

PMID: 31811768

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Project description:BackgroundBiosurfactants, being highly biodegradable, ecofriendly and multifunctional compounds have wide applications in various industrial sectors including environmental bioremediation. Surfactin, a member of lipopeptide family, which is considered as one of the most powerful biosurfactants due to its excellent emulsifying activities as well as environmental and therapeutic applications. Therefore, the aim of this study was to investigate the newly isolated bacterial strain S2MT for production of surfactin-like biosurfactants and their potential applications for oil-contaminated soil remediation.ResultsIn this study, the strain S2MT was isolated from lake sediment and was identified as Bacillus nealsonii based on transmitted electron microscopy (TEM) and 16S rRNA ribo-typing. The strain S2MT produced biosurfactant that reduced the surface tension (34.15 ± 0.6 mN/m) and displayed excellent emulsifying potential for kerosene (55 ± 0.3%). Additionally, the maximum biosurfactant product yield of 1300 mg/L was achieved when the composition of the culture medium was optimized through response surface methodology (RSM). Results showed that 2% glycerol and 0.1% NH4NO3 were the best carbon/nitrogen substrates for biosurfactant production. The parameters such as temperature (30 °C), pH (8), agitation (100 rpm), NH4NO3 (0.1%) and NaCl (0.5%) displayed most significant contribution towards surface tension reduction that resulted in enhanced biosurfactant yield. Moreover, the extracted biosurfactants were found to be highly stable at environmental factors such as salinity, pH and temperature variations. The biosurfactants were characterized as cyclic lipopeptides relating to surfactin-like isoforms (C13-C15) using thin-layer chromatography (TLC), Ultra high performance liquid chromatography and mass spectrometry (UHPLC-MS). The crude biosurfactant product displayed up to 43.6 ± 0.08% and 46.7 ± 0.01% remediation of heavy engine-oil contaminated soil at 10 and 40 mg/L concentrations, respectively.ConclusionPresent study expands the paradigm of surfactin-like biosurfactants produced by novel isolate Bacillus nealsonii S2MT for achieving efficient and environmentally acceptable soil remediation as compared to synthetic surfactants.

Dataset Information

Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated Bacillus vallismortis MDU6 strain.

Publications

Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated <i>Bacillus vallismortis</i> MDU6 strain.

Similar Datasets

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