Epigenetic Modifiers Revamp Secondary Metabolite Production in Endophytic Nigrospora sphaerica.

ABSTRACT: During plant interaction, endophytes provide benefits to the host plant. Endophytes also contribute a variety of structural attributes with biological potential. Nigrospora sphaerica, which produces phomalactone from Adiantum philippense L., was subjected to epigenetic modification. High-performance liquid chromatography (HPLC) and Gas chromatography-mass spectrometry (GCMS) analysis were used to determine secondary metabolite profiling. Epigenetic modifiers like DNA Methyltransferase (DNMT) and Histone deacetylase (HDAC) inhibitors increased the expression of biosynthetic pathways. The activation of new metabolites was observed as a result of the activation of cryptic biosynthetic gene clusters, as well as the silencing of phomalactone in some treatments. When compared to DN

SUBMITTER: Ramesha KP 

PROVIDER: S-EPMC8678036 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature