Transcriptomic Changes Within Human Bone Marrow After Severe Trauma.

ABSTRACT:

Background

Severe trauma is associated with severe systemic inflammation and neuroendocrine activation that is associated with erythroid progenitor growth suppression and refractory anemia. Although distinct transcriptional profiles have been detected in numerous tissue types after trauma, no study has yet characterized this within the bone marrow. This study sought to identify a unique bone marrow transcriptomic response following trauma.

Methods

In a prospective observational cohort study, bone marrow was obtained from severely injured trauma patients with a hip or femur fracture (n = 52), elective hip replacement patients (n = 33), and healthy controls (n = 11). RNA was isolated from bone marrow using a Purelink RNA mini kit. Direct quantification of mRNA copies was perfo

SUBMITTER: Kelly LS 

PROVIDER: S-EPMC8678139 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature