Therapeutic Potential of B-1a Cells in Intestinal Ischemia-Reperfusion Injury.

ABSTRACT:

Background

Acute mesenteric ischemia is a common surgical emergency. Restoration of blood flow is a critical objective of treating this pathology. However, many patients suffer from ischemia-reperfusion (I/R) injuries at the time of revascularization, requiring prolonged hospitalizations. B-1a cells are a subtype of B lymphocytes with roles in regulating inflammation and tissue injury by spontaneous release of natural IgM and IL-10. We hypothesized that treatment with B-1a cells protects mice from intestinal I/R.

Methods

Mesenteric ischemia was induced in mice by placing a vascular clip on the superior mesenteric artery for 60 minutes. At the time of reperfusion, B-1a cells or PBS control were instilled into the peritoneal cavity (PerC) of mice. PerC lavage, blood, intestine

SUBMITTER: Royster W 

PROVIDER: S-EPMC8678159 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature