High-throughput characterization of mutations in genes that drive clonal evolution using multiplex adaptome capture sequencing.

ABSTRACT: Understanding how cells are likely to evolve can guide medical interventions and bioengineering efforts that must contend with unwanted mutations. The adaptome of a cell-the neighborhood of genetic changes that are most likely to drive adaptation in a given environment-can be mapped by tracking rare beneficial variants during the early stages of clonal evolution. We used multiplex adaptome capture sequencing (mAdCap-seq), a procedure that combines unique molecular identifiers and hybridization-based enrichment, to characterize mutations in eight Escherichia coli genes known to be under selection in a laboratory environment. We tracked 301 mutations at frequencies as low as 0.01% and inferred the fitness effects of 240 of these mutations. There were distinct molecular signatures of selectio

SUBMITTER: Deatherage DE 

PROVIDER: S-EPMC8678185 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature