Single-molecule mechanical fingerprinting with DNA nanoswitch calipers.

ABSTRACT: Decoding the identity of biomolecules from trace samples is a longstanding goal in the field of biotechnology. Advances in DNA analysis have substantially affected clinical practice and basic research, but corresponding developments for proteins face challenges due to their relative complexity and our inability to amplify them. Despite progress in methods such as mass spectrometry and mass cytometry, single-molecule protein identification remains a highly challenging objective. Towards this end, we combine DNA nanotechnology with single-molecule force spectroscopy to create a mechanically reconfigurable DNA nanoswitch caliper capable of measuring multiple coordinates on single biomolecules with atomic resolution. Using optical tweezers, we demonstrate absolute distance measurements with ån

SUBMITTER: Shrestha P 

PROVIDER: S-EPMC8678201 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature