Serine-threonine Kinase Receptor-Associated Protein is a Critical Mediator of APC Mutation-Induced Intestinal Tumorigenesis Through a Feed-Forward Mechanism.

ABSTRACT:

Background & aims

Inactivation of the Apc gene is a critical early event in the development of sporadic colorectal cancer (CRC). Expression of serine-threonine kinase receptor-associated protein (STRAP) is elevated in CRCs and is associated with poor outcomes. We investigated the role of STRAP in Apc mutation-induced intestinal tumor initiation and progression.

Methods

We generated Strap intestinal epithelial knockout mice (Strap^ΔIEC) by crossing mice containing floxed alleles of Strap (Strap^fl/fl) with Villin-Cre mice. Then we generated Apc^Min/+;Strap^fl/fl;Vill-Cre (Apc^Min/+;Strap^ΔIEC) mice for RNA-sequencing analyses to determine the mechanism of function of STRAP. We used human colon cancer cell lines (DLD1

SUBMITTER: Vu T 

PROVIDER: S-EPMC8678216 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature